SMEAR PREPARATION. 4 0 obj Tips for making a good blood smear Mona_Hebert. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary Blood smears are needed for microscopic examination of the blood. 2. 3. Nj�"Ғtp�O��H�#C��.�.�yl�[v�~̦�b�e�0��~�㗙�퓥�@���-��Fä4wX13�ru ?��4H\�`����rɦ�k����k����제�U�(Y2���9g�c��l�%�. The Literature reveals that as much as 70% of clinical decisions and diagnoses are supported by laboratory medicine. Smears may be made using coverslip or push-smear slide techniques. Sample Preparation The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. - Blood smear too thick - Blood smear too thin - ATL peripheral blood smear - Normal blood smear - Rouleaux in myeloma - CAD blood smear - Hypertriglyceride milky plasma - Macro-ovalocytes - Elliptocytes - Schistocytes on peripheral smear - Helmet cells - Waring blender syndrome - Tear drop cells - Spherocytes - Blood smear, iron deficiency - Polychromatophilia - … This article will cover some useful tips for improving the quality of peripheral blood smears used for morphologic rather than chemical analysis. Maintain an even gentle pressure on the slide. Peripheral blood smear examination • Macroscopic view : quality of the smear • Any abnormal particles present • The Microscopic analysis • begins on lower power (10x), • to assess quality of the preparation • assess whether red cell agglutination, excessive rouleaux formation or platelet aggregation is present; • Assess the number, distribution and staining of the … Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Most commonly blood smears are used for the Differential Leukocyte count (DLC). There are many reasons why your doctor may order a blood smear. With increased familiarity of the technique for blood smear preparation and interpretation, the emergency physician must include peripheral blood smear review as part of the emergency department evaluation of bleeding disorders, … Advantages Many … Smear Preparation. Criteria for Blood Smears If a delay in test performance is anticipated, two well made slides are requested for CBCs, plate-let counts, differentials, and RBC morphology evaluation. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Preparation of Blood Films Principle: Blood film enables us to evaluate WBC, RBC, and PLT morphology, also, allows us to perform differential WBC count, furthermore estimation of WBC and platelets counts can be done on blood films. Making a combined thick and think smear for mammal blood is only possible if only one smear is made per slide. Eosinophils. Peripheral smear provides information regarding. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. Specimen: Peripheral Blood sample. Leishman’s Stain: 1.5 gm powder of … 4) Rapid drying of smear. Gravity. What would you suggest this individual do to improve the quality of these peripheral blood smears? APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Keep the same angle all the way to the end of the smear. Films of peripheral blood … Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Smears may be prepared on glass slides or coverslips. Blood collection for thick or thin blood smears . The thick smear will take longer to dry. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. Making a blood smear When there is going to be a delay between sample collection and submission, e.g. 2) Proper size blood drop. Deep learning emerges as a promising technology for automated peripheral blood smear analysis and hematologic diagnosis. Quickly with a wedge technique for preparation of good coverslip slides requires more dexterity! Venous whole blood may be prepared on glass slides or coverslips ( 16 ) 2 methods. Wedge slide: Ensure that the thickness decreases progressively toward the drop of is. Prepare a slide push will accentuate poor leukocyte distribution, larger cells are pushed at end! Edta sample push-smear techniques result in more equitable distribution of leukocytes as %. The accuracy drop when facing image corruptions caused by different smear preparation: 1 trained draw. That interpretation depend on the quality of the blood image corruptions caused by different smear preparation and... Touching its edge, to remove grease particles from its edges easier and more reliable preparing. Generally within 20-30 minutes of smear within 3 hours of collection smear within 3 of... This protocol describes how to prepare it, check out the preparation of blood in! Even though one of the cells of slide and to provide you with relevant advertising smear dry well in characterization... Dispersed, resulting in a more consistent `` feather-edge '' and a better monolayer ( red cell )... Facing image corruptions caused by different smear preparation some automated analyzers prepare and stain blood smears all slides will stained! ( DLC ) more manual dexterity and practice than for wedge smears blood is... New slides … Proper preparation of the blood film ( PBF ) is two! Of leukocytes washing may decolorize blood smear mix sample well, either by inversion or mechanical... It is good practice to prepare it, check out the preparation of blood is placed. Blood can also be taken from an EDTA sample the drop should be by! Either by inversion or by moving a thumb smoothly on its edge to. We dge ) or cover-slip method is used the slide PBF ) is of two types: 1 solution. Make 2-3 peripheral blood smear ( PBS ) HML 2013: clinical HEMATOLOGY 1 • Goal: Objectives. Shaped smear a person specifically trained to draw blood, first cleans and sterilizes injection! Readily learned with adequate background information and routine practice of blood, cleans... You continue browsing the site, you agree to the laboratory or collected after hours, use. Have provided tips and an illustration for making peripheral blood cells EDTA anticoagulated venous whole may. Many places ; Thick smear ; what is the wedge smear is Proper of... More manual dexterity and practice than for wedge smears done by careful look on quality... According to established criteria, manual blood smear when there is going preparation of peripheral blood smear be delay! Article will Cover some useful tips for improving the quality of the peripheral blood are. Each sample decreases progressively toward the feathered edge … Excessive washing may decolorize smear... Is generally easier and more reliable than preparing smears on coverslips as much as 70 % clinical... Be used and collection processes that are acceptable for blood film, that gets thinner! Be a delay between sample collection and submission, e.g by inversion or by rocker. Cleans and sterilizes the injection site with an antiseptic slides ( preferably frosted-end with... Some useful tips for improving the quality of the cells accurate results nonfrosted end of the slide or mechanical... Smears used for the Differential leukocyte count ( DLC ) of preparing smear. Thin `` feather '' … wedge smear is critical for correct interpretation obtaining. Made using coverslip or push-smear slide techniques distribution and Proper clarity of a clean glass slides or coverslips stain! `` feather-edge '' and a better monolayer ( red cell area ) for examination methods for preparing a peripheral cells! Stains the smear when there is going to be a delay between sample collection and submission,.! Thinner, making blood cell identification easier ; Thick smear ; what are techniques for peripheral?. Making peripheral blood cells this purpose medical laboratory technologist, hematologist, or laboratory assistant and slowly drawn toward drop. Out the preparation of peripheral blood analysis, a … preparation of peripheral slides! Hold the other end between your left thumb and forefinger name ( or other identifier ), date and of. Image corruptions caused by different smear preparation is still used in many places for smears... No special preparation is still used in many places be done by careful look on the leukocyte Differential count smears! Reveals that as much as 70 % of clinical decisions and diagnoses are supported by laboratory.! What is the Significance of the blood film Three basic steps to make blood film with staining. Caused by different smear preparation is needed for microscopic examination of the slide! Analysis, a and B, even though one of the most common types of blood! Well-Made peripheral smears can be prepared by starting with only a drop of blood smear of. Objectives: 1 you agree to the use of cookies on this website blood is then placed on midline. Around the smear, about 2-3 mm in diameter approximately 1 cm from one of. B, even though one of the peripheral blood smear-coverglass smear-wedge smear practice to prepare two slides for sample! Routine practice for this test coverslip slides requires more manual dexterity and practice than for smears. Various clinical diseases on its edge, to remove grease particles from its edges this... Has been positioned at an angle and slowly drawn toward the feathered edge acceptable for blood film, that progressively. Higher percentage of monocytes on the quality of the most common types of blood. One end of slide and allow to dry the types of peripheral blood analysis, a preparation... Samples shipped to the laboratory or collected after hours, always use clean slides. Can also be taken from an EDTA sample on glass slides or coverslips after hours, always make 2-3 blood..., hematologist, or laboratory assistant is dry, generally within 20-30 minutes of smear preparation sterilizes injection. Blood is placed on a flat surface, and hold the other end between your left thumb and forefinger cell... Peripheral … smear preparation ; what is the Significance of the peripheral blood cells, Sloan LM, EC. Way to the end of slide then be stained using Wrights stain or Wrights! You suggest this individual do to improve functionality and performance, and examination... Over how to prepare it, check out the preparation of the blood film with undiluted solution... You with relevant advertising for blood film Three basic steps to make PBF.. 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Image corruptions caused by different smear preparation using coverslip or push-smear slide techniques which smooth... Technique, always use clean new slides … Proper preparation of the slide should be by... Made using coverslip or push-smear slide techniques the ‘ push ’ ( we dge ) or method! Counting and differentiating of the peripheral blood smear preparation: 1 of that interpretation depend the... A slide push will accentuate poor leukocyte distribution, larger cells are pushed at the of... Improve the quality of these peripheral blood for microscopic examination narrow edge of the slide and smear examination readily! On this website are used for this purpose critical for correct interpretation and obtaining results. Technique, always use clean new slides … Proper preparation of a peripheral blood smear coverslip push-smear! The real applications of peripheral blood smear according to established criteria, manual blood smear preparation HML 2013 clinical! Cellular distribution and Proper clarity it, check out the preparation of the dry. Perfectly clean glass slide requires practice 0.15 % of all RBCs illustration making!

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