To Outline the Proper Procedures for Histopathology . 3. 5. Clearing c. Infilteration 7. Deparaffinize and hydrate to distilled water. A number of histologic stains are available that are routinely used to visualize fungi in tissue sections. 1. II. Open biopsies are sent fresh on saline dampened gauze. 18 Record of Changes Histology Procedure Fixation 5. This textbook deals with basic histopathology techniques: Introduction and examination of tissue, fixation and fixatives, tissue processing and … Wash in tap water, rinse in distilled 3 changes. 4. Wash in tap water, rinse in distilled. HISTOLOGY ATC BRANCH HD-19-HSC-10.2 HISTOLOGY SPECIMEN COLLECTION Effective: 2/18/13, Rev 3/4/2014 9. 1. To outline the proper procedures for collecting, preserving, identifying, and processing tissue specimens for evaluation by light microscopic histopathological evaluation. 9 HISTOPATHOLOGY LABORATORY Histopathology laboratory is a unit performing clinical activity associated with diagnosis during and post-surgery. 6. PROCEDURE: 1. 2. Histology Procedure Fixation Tissue Processing a. Dehydration b. 1% Sodium metabisulfite, 1 minute, room temperature. histopathology techniques. 5.7 Staining procedures Whenever doing staining procedures ensure that protective gowns, gloves and safety glasses are worn. The lab examines tissue materials, based on which an emergency diagnosis is needed during surgery (i.e. RESPONSIBILITY . When ‘bringing sections to water’ or ‘dehydrating, clearing and mounting’ always reagents used within the histology laboratory are available both within the laboratory and in the preparation room (C222). You may modify each section in the space provided at the bottom of each page. HISTOLOGY AND CYTOLOGY 51 MODULE Microtome Histology and Cytology Notes Fig. *2% Chromic acid, microwave Hi power, 45 seconds, allow to stand for 5 minutes. procedure or unexpected findings Example: “possible positive deep margin” or “possible neoplasm” o Place the Pathology Tissue Slip in the side pocket of the biohazard bag along with the insurance information sheet • For specimens ordered online: 1. Muscle Biopsies (for detailed instructions see Clinical Guidelines, GOS Web) All muscle biopsies should be pre-booked with histopathology. 9.2: Principle of sample movement for making a cut on a rotary microtome In the figure to the left, the principle of the cut is explained. Choose appropriate inpatient surgical request card or outpatient histopathology specimen requisition and fill in completely (if applicable). a. 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